Changes in Lectin-Binding Features of Ram Sperm Surfaces Associated with Epididymal Maturation and Ejaculation1
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چکیده
منابع مشابه
Changes in calmodulin level and cAMP-dependent protein kinase activity during epididymal maturation of ram spermatozoa.
Calmodulin concentration and cAMP-dependent protein kinase activity were simultaneously determined on ram spermatozoa collected by cannulation of successive segments of the epididymal tubule. Epididymal transit was characterized on one hand by an overall decrease in the calmodulin level and on the other by a dramatic rise in the cAMP-dependent protein kinase activity. In contrast to the calmodu...
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Mammalian sperm contain the serine/threonine phosphatases PP1γ2 and PP2A. The role of sperm PP1γ2 is relatively well studied. Here we confirm the presence of PP2A in sperm and show that it undergoes marked changes in methylation (leucine 309), tyrosine phosphorylation (tyrosine 307) and catalytic activity during epididymal sperm maturation. Spermatozoa isolated from proximal caput, distal caput...
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MODIFICATIONS IN RABBIT SPERM PLASMA MEMBRANES DURING EPIDIDYMAL PASSAGE AND AFTER EJACULATION WERE INVESTIGATED BY USED OF THREE LECTINS: concanavalin A (Con A); Ricinus communis I (RCA(I)); and wheat germ agglutinin (WGA). During sperm passage from caput to cauda epididymis, agglutination by WGA drastically decreased, and agglutination by RCA(I) slightly decreased, although agglutination by C...
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Ram spermatozoa from the distal caput, corpus, and cauda epididymis and ejaculated spermatozoa were artificially inseminated in 170 ewes which had been treated with an ovulatory dose of PMSG 61 ! 4 hrs previously. Results were expressed as the percent of ewes pregnant 18 days after insemination (F = with positive progesterone assay/ Q inseminated) and embryonic survival (ES = ewes lambing/ preg...
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One of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. The four pattern recognition molecules that have been identified till now are Mannose binding lectin (MBL), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. MBL ...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1988
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod38.3.667